Ticipated in its design and coordination as well as drafted the > 자유게시판

Ticipated in its design and coordination as well Marimastat as drafted the manuscript. All authors have read and approved the final version of the manuscript.Protein samples were resolved on 12 SDS-polyacrylamide gels and transferred to Osmonics nitrocellulose membrane. Resolved proteins were detected by their specific primary antibodies and horseradish peroxidaseconjugated secondary antisera. The immunoblots were visualized by chemiluminescence with the ECL kit from Amersham, and the images detected in X-ray films were quantified by densitometric scanning using the Eagle Eye II still video system (Stratagene, La Jolla, CA, USA).Kwan et al. BMC Structural Biology (2015) 15:Page 14 ofAcknowledgements We thank Andy Liu, Cecilia Wong, and Joy Chan for helpful discussions. This work was supported in part by the National Key Basic Research Program of China (2013CB530900), Research Grants Council of Hong Kong (HKUST 660109), and the Hong Kong Jockey Club. This work was supported in part by the National Key Basic Research Program of China (2013CB530900), Research Grants Council of Hong Kong (HKUST 660109), and the Hong Kong Jockey Club. Received: 24 July 2015 Accepted: 26 AugustReferences 1. Oldham WM, Hamm HE. Heterotrimeric G protein activation by G-protein-coupled receptors. Nat Rev Mol Cell Biol. 2008;9(1):60?1. 2. Tesmer JJ. The quest to understand heterotrimeric G protein signaling. Nat Struct Mol Biol. 2010;17(6):650?. 3. Sandal M, Paltrinieri D, Carloni P, Musiani F, Giorgetti A. Structure/function relationships of phospholipases C Beta. Curr Protein Pept Sci. 2013;14(8):650?. 4. Jhon DY, Lee HH, Park D, Lee CW, Lee KH, Yoo OJ, et al. Cloning, sequencing, purification, and Gq-dependent activation of phospholipase C-beta 3. J Biol Chem. 1993;268(9):6654?1. 5. Lyon AM, Tesmer JJ. Structural insights into phospholipase C- function. Mol Pharmacol. 2013;84(4):488?00. 6. Adjobo-Hermans MJ, Crosby KC, Putyrski M, Bhageloe A, van Weeren L, Schultz C, et al. PLC isoforms differ in their subcellular location and their CT-domain dependent interaction with Gq. Cell Signal. 2013;25(1):255?3. 7. Berridge MJ, Irvine RF. Inositol trisphosphate, a novel second messenger in cellular signal transduction. Nature. 1984;312:315?1. 8. Ross E, Wilkie T. GTPase-activating proteins for heterotrimeric G proteins: regulators of G protein signaling (RGS) and RGS-like proteins. Annu Rev Biochem. 2000;69:795?27. 9. Dorn GW, Oswald KJ, McCluskey TS, Kuhel DG, Liggett SB. 2A-adrenergic receptor stimulated calcium release is transduced by Gi-associated Gmediated activation of phospholipase C. Biochemistry. PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/9221828 1997;36:6415?3. 10. Park D, Jhon DY, Lee CW, Lee KH, Rhee SG. Activation of phospholipase C isozymes by G protein subunits. J Biol Chem. 1993;268:4573?. 11. Rozengurt E. Mitogenic signaling pathways induced by G proteincoupled receptors. J Cell Physiol. 2007;213(3):589?02. 12. Gaugler L, Raamsdonk CD V, Bezrookove V, Green G, Brien JMO, Simpson EM, et PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/18218841 al. Frequent somatic mutations of GNAQ in uveal melanoma and blue naevi. Nature. 2009;457(7229):599?02. 13. Waldo GL, Ricks TK, Hicks SN, Cheever ML, Kawano T, Tsuboi K, et al. Kinetic scaffolding mediated by a phospholipase C and Gq signaling complex. Science. 2010;330:974?0. 14. Lyon AM, Dutta S, Boguth CA, Skiniotis G, Tesmer JJG. Full-length Gqphospholipase C-3 structure reveals interfaces of the C-terminal coiled-coil domain. Nat Struct Mol Biol. 2013;20(3):355?2. 15. Park D, Jhon DY, Lee CW, Ryu SH, Rhee SG. Removal of th.