Avy chain 1 and anti-Rac1 antibodies (see below).Quantitative proteome analysis of > 자유게시판

Avy chain 1 and anti-Rac1 antibodies (see below).Quantitative proteome analysis of Caco-2 cells treated with rTcdA wt and mutant rTcdA, respectively, for 24 hFigure 3 rTcdA glucosylates Rac-1 in Caco-2 cells. The cytopathic effect was shown by a Rac-1 glucosylation sensitive western blot analysis. The used antibody did not bind to glucosylated Rac-1. 1 and 4: control cells; 2 and 5: rTcdA wt treated cells; 3 and 6: mutant rTcdA treated cells.Cytosolic proteins from control cells and cells treated with either rTcdA wt or mutant rTcdA were labeled with light, heavy, Staurosporine and medium ICPL-reagent, respectively. Two technical replicates of the first biological replicate and one technical replicate from the second biological replicate were prepared and analyzed. 485 proteins were identified by at least two unique peptides, 420 proteins were quantified by at least one unique peptide and 286 proteins were quantified by at least two unique peptides. The false discovery rates for all replicates were 1.05 , 1.16 and 0.83 with p PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/16989806 ANXA3 GFPT1 MIF PRDX2 PRDX1 Number of peptides for quantitation 8 3 5 4 6 10 6 regulation factor1 2.39 1.96 1.59 1.79 1.59 1.75 1.76 SD2 T-test3 0.53 0.0.31 <0.0001 0.22 0.51 0.35 0.36 0.14 0.015 0.005 0.042 0.001 0.10 Heterogeneous nuclear ribonucleoprotein A1 11 Polypyrimidine tract-binding protein 1 12 Glucosidase 2 subunit alpha 13 Glucosidase 2 subunit beta 14 Endoplasmin 15 Protein disulfide-isomerase 16 Protein disulfide-isomerase A4 after treatment with mutant rTcdA, 24 h Nr. Protein name 1 Filamin A after treatment with rTcdA wt, 24 h Nr. Protein name 1 2 3 4 5 6 7 8 9 Actin, cytoplasmic 1 Filamin A Filamin B Villin-1 Apolipoprotein E Creatine kinase B-type Glucosidase 2 subunit beta Peroxiredoxin-1 Peroxiredoxin-0.13 <0.0001 0.04 <0.0001 0.28 0.008 0.3 0.Accession number P21333 Accession number P60709 P21333 O75369 P09327 P02649 P12277 P14314 Q0.